Differentiation and Malignant Transformation of Epithelial Cells: 3D Cell Culture Models
Sinikka Eskelinen, Ph.D.
Biocenter Oulu and Unit of Cancer Research and Translational Medicine, Faculty of Medicine, University of Oulu
P.O.Box 5000, FIN-90014 University of Oulu, Finland
Aapistie 5A, FIN-90220 Oulu, Finland
e-mail: sinikka.eskelinen (at) oulu.fi
Project leader biography
I am a cell biologist and biophysicist in training. Currently I am working as the research director of the University of Oulu, Finland, as one of the duties the scientific evaluations of the research institutions of the University and the general research assessment exercise of the whole University of Oulu. I am still running my research group at Biocenter Oulu. In my group we are investigating the cell signaling pathways regulating the actin-based cytoskeleton and cell - cell junctions in epithelial cells and carcinoma cell lines. We aim to understand the mechanisms behind the maintenance of differentiated phenotype of cultured epithelial cells and the mechanisms how the polarity programs are changed during malignant transformation to cancer cells. I am also in charge of the light microscopy core facility of the Biocenter Oulu Tissue Imaging Center which is a member of Oulu BioImaging (OBI) network, National Imaging Infrastructure Network (NIIN) of Biocenter Finland and an associate member of Euro-BioImaging (ESFRI) network.
About the project
We aim to understand the mechanisms behind the maintenance of a differentiated phenotype and analyse the electrophysiological parameters which regulate the transport capacity of kidney epithelial cells. We also aim to understand the circumstances in which polarity programs are changed during malignant transformation to cancer cells. We use non-transformed and Src-transformed kidney epithelial MDCK cells as models. On the basis of the results we will try to find pharmacologically active compounds regulating secretion or acting as tumour suppressors in cell culture environments, and draw conclusions concerning their capacity to do the same in vivo.
Last updated: 13.10.2016